Increasing fluorescence

General information:

The amplification of weak fluorescence signals is still challenging. Here we present an AlSiO₂ coated microscopic slide, that enhances fluorescence signals up to four times. It is essential to place specimen directly on the AlSiO₂ layer. These new commercially available microscopic slides can be used for conventional fluorescence microscopy as well as for confocal laser scan microscopy.

 

Sensitivity and Specificity:

 

We observed an increasing of the fluorescence signal up to four times in comparison to a conventional transparent microscopic slide. The data analysis was performed as a semi-quantitative evaluation with the help of XIMEA camera-software (XIMEA API Software Package V4, Version 4.20, 64-bit, XIMEA, Münster, Germany) and ImageJ (ImageJ 1.52a, National Institutes of Health, USA).

 

Run your samples on Cytecs INVERSO

 

 

 

 

Addional information:

AlSiO₂ Schicht erhöht die Photonenausbeute in der Fluoreszenzmikroskopie

 

Literature:

Takeshi Sudo, Masayuki Mizusawa, Vertical illumination type fluorescence microscope, Japanese Patent Office 09292572 A, 11.11.1997

Triantafyllos Tafas, System and method for increasing the contrast of an image produced by an epifluorescence microscope, US 2006/0056016 A1, 16, März 2006

Wolfgang Göhde, Objektträger für die Fluoreszenz-Auflicht-Mikroskopie, DE20 2022 101 038 U1, Mai 2022