CD4 count kit
Quantity: 100 Tests
Antibody: monoclonal anti human CD4
IT4-R-PE mouse IgG1
Application: Flow cytometry
(λ=488nm or λ=532nm)
Liquid antibody kit for counting of CD4 positive lymphocytes for monitoring of HIV/ AIDS patients
International efforts are on the way to improve the follow-up diagnostic for HIV/AIDS patients by replacing CD4 immunodiagnostic by viral load analysis techniques.
The implementation of wide spread and point of care laboratory capacity for viral load analysis is on the way and may last some more years. This means that CD4 (Greve B. et al., 2003; Pattanapanyasat K., et al. 2005) and CD4% (Greve B. et al., 2004) counting will remain as the key diagnostic tool for several years to come.
Since about 15 years great effort has been invested in order to improve CD4 techniques and to develop new protocols with the aim to make this indispensable diagnostic test affordable and available to everybody who needs it.
For further improvement of the quality of the CD4 test results we introduce a protocol, which can be used for all flow cytometers.
Greve B., Göhde R.; New Perspectives for Monitoring HIV-infected Patients in Developing Countries by Affordable CD4+ T-cell Counts; Business Briefing: Long-Term Healthcare Strategies 2003
Pattanapanyasat K., Lerdwana S., Noulsri E., Chaowanachan T., Wasinrapee P., Sakulploy N., Pobkeeree V., Suksripanich O., Thanprasertsuk S., Spira T. J., Tappero J. W., Levine W. C.; Evaluation of a new single-parameter volumetric flow cytometer (CyFlow(green)) for enumeration of absolute CD4+ T lymphocytes in human immunodeficiency virus type 1-infected Thai patients; Clin Diagn Lab Immunol. 2005 Dec;12(12):1416-24
Greve B., Göhde W., Cassens U., Göhde R.; New Protocols to Follow Up HIV-induced Immune Impairment in Paediatric Samples; Business Briefing:
Long-Term Healthcare Stratregies 2005
Cassens U., Greve B., Tapernon K., Nave B., Severin E., Sibrowski W., Göhde W.; A novel true volumetric method for the determination of residual leucocytes in blood components; Vox Sang. 2002 May;82(4):198-206
Greve B., Cassens U., Westerberg C., Göhde W. jun., Sibrowski W., Reichelt D., Göhde W.; A New No-Lyse, No-Wash Flow-Cytometric Method for the Determination of CD4 T Cells in Blood Samples; Transfus Med Hemother 2003;30:8–13; (DOI:10.1159/000069339)